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Image Search Results
Journal: Cancer Cell International
Article Title: Effect of Matrine on HPAC cell migration by down-regulating the expression of MT1-MMP via Wnt signaling
doi: 10.1186/s12935-015-0210-4
Figure Lengend Snippet: The migration of HPAC cells was inhibited by Matrine. Log-phase cells were treated with normal complete RPMI-1640 alone or contained with 50 μg/ ml Matrine or 0.05 μg/ ml Docetaxel ( a ). Data were expressed as mean ± S.E.M from three separated experiments ( b ). Cell invasion ability was detected by transwell assay ( c ). Statistical analyses was performed using the t -test. * ( P < 0.05) indicates a significant difference compared with the control group
Article Snippet: Other reagents used in this study, such as Anti-mouse-IgG-HRP and Anti-Rabbit-IgG-HRP, were purchased from California Bioscience (California Bioscience, USA),
Techniques: Migration, Transwell Assay, Control
Journal: International Journal of Molecular Medicine
Article Title: LRP6 is involved in the proliferation, migration and invasion of trophoblast cells via miR-346
doi: 10.3892/ijmm.2020.4570
Figure Lengend Snippet: Overexpression of LRP6 enhances migration and invasion of trophoblast cells. Negative control (siNC) and siLRP6 were transfected into JEG-3 cells, whereas HTR8/SVneo cells were transfected with LRP6 or empty pcDNA3.1 vector (Mock), and un-transfected cells served as a control for comparison. (A-F) Microscopic images and quantitative analysis of wound healing and Transwell assays in cells following transfection. (G-L) Western blot analysis and RT-qPCR analyses were performed to determine the expression levels of genes and proteins associated with migration and invasion (i.e., MMP-2, MMP-9, TIMP-1, TIMP-2). The magnification of the images (either x100 or ×200) is indicated in the Figure parts, where applicable. ** P<0.001, * P<0.05 vs. siNC or Mock (n=3). ns, not significant; LRP6, low-density-lipoprotein receptor-related protein 6; MMP, matrix metalloproteinase; TIMP, tissue inhibitor of metal-loproteinase.
Article Snippet: The invasive abilities of the transfected cells were determined using Costar™
Techniques: Over Expression, Migration, Negative Control, Transfection, Plasmid Preparation, Western Blot, Quantitative RT-PCR, Expressing
Journal: International Journal of Molecular Medicine
Article Title: LRP6 is involved in the proliferation, migration and invasion of trophoblast cells via miR-346
doi: 10.3892/ijmm.2020.4570
Figure Lengend Snippet: Downregulated miR-346 promotes migration and invasion of trophoblast cells. Mimics control, miR-346 mimics, inhibitor control, and miR-346 inhibitor were respectively transfected into the HTR8/SVneo and JEG-3 cells. Microscopic images and quantitative analysis of (A-D) wound healing and (E-H) Transwell assays in cells following transfection. The magnification of the images (either x100 or x200) is indicated in the Figure parts, where applicable. ** P<0.001; * P<0.05, vs. mimics control; ## P<0.001; # P<0.05 vs. inhibitor control (n=3).
Article Snippet: The invasive abilities of the transfected cells were determined using Costar™
Techniques: Migration, Transfection
Journal: International Journal of Molecular Medicine
Article Title: LRP6 is involved in the proliferation, migration and invasion of trophoblast cells via miR-346
doi: 10.3892/ijmm.2020.4570
Figure Lengend Snippet: LRP6 reverses the inhibitory effect of miR-346 on the activity, migration and invasion of trophoblast cells. JEG-3 cells were transfected with inhibitor control, miR-346 inhibitor, inhibitor control + mock, and inhibitor + siLRP6, whereas HTR8/SVneo cells were transfected with mimics control, miR-346 mimics, mimics control + mock, and mimics + LRP6. (A and B) CCK-8 assays were performed to detect the cell activity following transfection. Microscopic images and quantitative analysis of (C-F) wound healing and (G-J) Transwell assays in the cells following transfection. The magnification of the images (either x100 or x200) is indicated in the Figure parts, where applicable. ** P<0.001; * P<0.05 vs. inhibitor control or mimics control; ## P<0.001; # P<0.05 vs. inhibitor or mimics (n=3).
Article Snippet: The invasive abilities of the transfected cells were determined using Costar™
Techniques: Activity Assay, Migration, Transfection, CCK-8 Assay